Amazing Discoveries of Benthic Fauna from the Abyssal Zone of Lake Baikal.

Lake Baikal is a natural laboratory for the study of species diversity and evolution, as a unique freshwater ecosystem meeting the all of the main criteria of the World Heritage Convention. However, despite many years of research, the true biodiversity of the lake is clearly insufficiently studied, especially that of deep-water benthic sessile organisms. For the first time, plastic waste was raised from depths of 110 to 190 m of Lake Baikal. The aim of this study was to examine the biological community inhabiting the plastic substrate using morphological and molecular genetic analysis. Fragments of plastic packaging materials were densely populated: bryozoans, leeches and their cocoons, capsules of gastropod eggs, and turbellaria cocoons were found. All the data obtained as a result of an analysis of the nucleotide sequences of the standard bar-coding fragment of the mitochondrial genome turned out to be unique. Our results demonstrate the prospects for conducting comprehensive studies of artificial substrates to determine the true biodiversity of benthos in the abyssal zone of Lake Baikal.

The Phylogenetic Position of Spironucleus sp. (Diplomonadida: Hexamitidae) from the Intestine of Chinese Sleeper Perccottus glenii Dybowski, 1877 (Actinopterygii: Odontobutidae).

INTRODUCTION: The Chinese (Amur) sleeper (Perccottus glenii Dybowski, 1877) (Actinopterygii: Odontobutidae) is a freshwater fish species with high invasive potential. Diplomonads have been detected in the intestines of Chinese sleepers using light microscopy. AIM: The aim of this study was to identify the diplomonads in Chinese sleepers using molecular-genetic methods. MATERIALS AND METHODS: The fish used in this analysis were caught in the following bodies of water in Russia between 2014 and 2016: Lake Dolgoe, the floodplain of the Ingoda River (Amur River basin), the Tsna River (the Oka River basin), and the littoral of the Kotlin Island (Gulf of Finland). Partial sequences of small subunit rRNA genes were obtained for the intestinal diplomonads of Chinese sleeper. RESULTS: The analysis of all sequenced samples revealed the presence of Spironucleus salmonis Moore, 1922; other Spironucleus species were not found in the sampled fish. With 82% probability, the sampled sequences of diplomonads from Chinese sleeper formed a separate cluster in the clade of S. salmonis on the phylogenetic tree. CONCLUSION: This is the first record of S. salmonis in fish in the family Odontobutidae.

Genetic diversity of Diplomonadida in fish of the genus Coregonus from Southeastern Siberia.

Diplomonadida are primitive flagellate protozoa, among which both commensals and pathogens have been recorded. To date, members of the genera Hexamita and Spironucleus have been reported in the digestive system of fish in the Baikal region. We determined the genetic diversity of Diplomonadida in fish of the genus Coregonus from south-eastern Siberia using molecular-genetic methods. Fish for analysis were caught in Lake Baikal and in the Barguzin, Nepa, Chechuy, and Kirenga rivers from 2010 to 2013. Gall bladders, hindguts and foreguts of 120 specimens of Coregonus migratorius representing three morpho-ecological groups, 25 specimens of Coregonus lavaretus baicalensis, 25 specimens of Coregonus tugun and 30 specimens of Coregonus lavaretus pidschian were analysed via amplification with primers specifically designed for eukaryotes. Amplicons positive for Diplomonadida were sequenced. A phylogenetic analysis revealed that diplomonad flagellates of whitefish from Southeastern Siberia belong to Spironucleus barkhanus. Positive Diplomonadida DNA samples were analysed with primers designed in the present study for the amplification of small subunits of ribosomal DNA fragments of S. barkhanus (about 1,430 bp) and sequenced. Phylogenetic analysis revealed inside the clade of S. barkhanus besides the cosmopolitan genotype from European salmon that was detected earlier in Baikalian grayling, a new genotype unique to the fish of the genus Coregonus from Lake Baikal.

Long-term cultivation of primmorphs from freshwater Baikal sponges Lubomirskia baikalensis.

The work was aimed at performing long-term cultivation of primmorphs in vitro from freshwater sponge Lubomirskia baikalensis (Pallas 1776), collected from Lake Baikal, obtaining its long-term primmorph culture in both natural (NBW) and artificial (ABW) Baikal water and at identifying the impact of different environmental factors on formation and growth of primmorphs. The first fine aggregates of L. baikalensis are formed in vitro 10-15 min after dissociation of sponge cells. Epithelization of aggregates begins 4 h later after the dissociation. Young primmorphs are formed 1 or 2 days later. The surface of primmorphs is covered with a layer of exopinacocytes. The primmorphs remain viable for more than 10 months at 3-6 °C. Over 50% of primmorphs in NBW and 25% in ABW are attached to the substrate and grow like adult sponges. Thus, the long-term primmorph cultivation in vitro allows the creation of a controlled live model system under experimental conditions. The results of this work will allow the creation of a cell culture collection of Baikal freshwater sponges for studying morphogenesis of primmorphs during cultivation at different stages and transdifferentiation of their cells, physiological functions of sponge cells, processes of spiculogenesis, identification of proteins involved in biomineralization process, decoding of their genes, as well as a spectrum of secondary metabolites.